Plant Phenotyping

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  1. Plant Phenotyping Server Configuration

This document details the recommended server configuration for running plant phenotyping software and associated data processing pipelines. This guide is intended for newcomers to the server administration aspects of our research environment. This setup balances performance, scalability, and data integrity. It assumes a baseline familiarity with Linux server administration and network concepts. Please consult with the System Administrators Group for any questions or concerns.

Overview

Plant phenotyping generates large volumes of data, including images, time-series measurements, and environmental data. A robust server infrastructure is critical for efficient data storage, processing, and analysis. This configuration focuses on a multi-tier architecture: data acquisition, data storage, and processing/analysis. We will primarily focus on the processing/analysis tier, assuming data acquisition servers are handled separately (see Data Acquisition Protocols). Data storage is covered in Data Storage Solutions. This document details the specifications for the processing server.

Server Hardware Specifications

The following table details the minimum and recommended hardware specifications for the plant phenotyping processing server. These specifications are designed to handle typical workloads, but may need adjustment based on specific project requirements. Consider Scalability Considerations when planning future upgrades.

Component Minimum Specification Recommended Specification
CPU Intel Xeon E5-2620 v4 (6 cores, 12 threads) Intel Xeon Gold 6248R (24 cores, 48 threads)
RAM 64 GB DDR4 ECC Registered 128 GB DDR4 ECC Registered
Storage (OS & Software) 500 GB SSD 1 TB NVMe SSD
Storage (Data Processing) 4 TB HDD (RAID 1) 8 TB HDD (RAID 5 or 10)
GPU NVIDIA GeForce RTX 3060 (12 GB VRAM) NVIDIA RTX A5000 (24 GB VRAM)
Network Interface 1 Gbps Ethernet 10 Gbps Ethernet

Software Stack

The following software stack is recommended for the plant phenotyping processing server.

  • Operating System: Ubuntu Server 22.04 LTS – Offers excellent stability, security updates, and package availability.
  • Programming Languages: Python 3.9 or higher, R 4.2 or higher.
  • Data Analysis Libraries: NumPy, SciPy, Pandas, Scikit-learn, OpenCV. These are typically managed using Anaconda.
  • Database: PostgreSQL 14 – For storing metadata and analysis results.
  • Workflow Management: Nextflow or Snakemake – For automating and managing complex processing pipelines.
  • Version Control: Git – For managing code and configuration files. Repository hosted on GitLab.
  • Containerization: Docker and Docker Compose – For creating reproducible environments.

Network Configuration

Proper network configuration is crucial for data access and communication. The server should have a static IP address and be accessible via SSH. Firewall rules should be configured to allow only necessary traffic. Refer to the Network Security Policy for detailed guidelines.

Setting Value
IP Address Static, assigned by Network Administration
Subnet Mask 255.255.255.0 (example)
Gateway Assigned by Network Administration
DNS Servers 8.8.8.8, 8.8.4.4 (Google Public DNS)
SSH Access Enabled, restricted to authorized users
Firewall UFW (Uncomplicated Firewall) enabled with appropriate rules

Data Processing Pipeline Configuration

The data processing pipeline is the core of the plant phenotyping workflow. It typically involves image processing, feature extraction, and statistical analysis. Pipelines should be designed to be modular and reproducible. Consider using workflow management systems like Nextflow or Snakemake to orchestrate the pipeline steps. Detailed documentation on pipeline development can be found on the Pipeline Development Wiki.

Pipeline Stage Description Software/Libraries
Image Preprocessing Noise reduction, contrast enhancement, and image registration. OpenCV, Scikit-image
Feature Extraction Identification and measurement of plant traits (e.g., height, area, color). OpenCV, PlantCV, custom Python scripts
Data Analysis Statistical analysis of extracted features. R, Pandas, SciPy, Scikit-learn
Data Visualization Creation of charts and graphs to visualize results. Matplotlib, Seaborn, R ggplot2

Security Considerations

Server security is paramount. Regularly update the operating system and software packages. Implement strong passwords and multi-factor authentication. Monitor system logs for suspicious activity. Consult the Security Best Practices document for detailed security guidelines. Data backups should be performed regularly and stored in a secure location (see Backup and Recovery Procedures).

Further Resources


Intel-Based Server Configurations

Configuration Specifications Benchmark
Core i7-6700K/7700 Server 64 GB DDR4, NVMe SSD 2 x 512 GB CPU Benchmark: 8046
Core i7-8700 Server 64 GB DDR4, NVMe SSD 2x1 TB CPU Benchmark: 13124
Core i9-9900K Server 128 GB DDR4, NVMe SSD 2 x 1 TB CPU Benchmark: 49969
Core i9-13900 Server (64GB) 64 GB RAM, 2x2 TB NVMe SSD
Core i9-13900 Server (128GB) 128 GB RAM, 2x2 TB NVMe SSD
Core i5-13500 Server (64GB) 64 GB RAM, 2x500 GB NVMe SSD
Core i5-13500 Server (128GB) 128 GB RAM, 2x500 GB NVMe SSD
Core i5-13500 Workstation 64 GB DDR5 RAM, 2 NVMe SSD, NVIDIA RTX 4000

AMD-Based Server Configurations

Configuration Specifications Benchmark
Ryzen 5 3600 Server 64 GB RAM, 2x480 GB NVMe CPU Benchmark: 17849
Ryzen 7 7700 Server 64 GB DDR5 RAM, 2x1 TB NVMe CPU Benchmark: 35224
Ryzen 9 5950X Server 128 GB RAM, 2x4 TB NVMe CPU Benchmark: 46045
Ryzen 9 7950X Server 128 GB DDR5 ECC, 2x2 TB NVMe CPU Benchmark: 63561
EPYC 7502P Server (128GB/1TB) 128 GB RAM, 1 TB NVMe CPU Benchmark: 48021
EPYC 7502P Server (128GB/2TB) 128 GB RAM, 2 TB NVMe CPU Benchmark: 48021
EPYC 7502P Server (128GB/4TB) 128 GB RAM, 2x2 TB NVMe CPU Benchmark: 48021
EPYC 7502P Server (256GB/1TB) 256 GB RAM, 1 TB NVMe CPU Benchmark: 48021
EPYC 7502P Server (256GB/4TB) 256 GB RAM, 2x2 TB NVMe CPU Benchmark: 48021
EPYC 9454P Server 256 GB RAM, 2x2 TB NVMe

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⚠️ *Note: All benchmark scores are approximate and may vary based on configuration. Server availability subject to stock.* ⚠️